Mark Hamill said this is the last time that he will be voicing the Joker, and Paul Dini the writer of the Batman Animated series and Arkham City did kill the Joker in the Batman Beyond movie.

Just because a voice actor stops doing a character, doesn't mean the character will get killed off.
Do you have any other reasoning behind killing the Joker?

I heavily doubt they would kill off the Joker.

Batman villains don't ever die do they ?

No, no one in the history of comics, villian or hero, has ever died; if they have, they came back.

No, no one in the history of comics, villian or hero, has ever died; if they have, they came back.

A non Mark Hamil Joker is not really "The Joker" as far as I'm concerned. So yes. The joker is permanently being killed as we know it. I certainly won't be taking interest in any other Joker related multimedia.

Mark Hamill also said Arkham Asylum was the last time he'd be doing Joker. You see how that went.

What do the events in a Batman Beyond movie have to do with Arkham Asylum? 

I doubt it. I'd even be willing to make a wager.

Mark Hamill also said Arkham Asylum was the last time he'd be doing Joker. You see how that went.

well that doesnt mean hes gonna die

I think you're wrong.

@NTM said:

No, no one in the history of comics, villian or hero, has ever died; if they have, they came back.

Ben Parker.

Batman villains don't ever die do they ?

Well it's not out of the question that he can't die, there has been quite a few Batman villians killed off but rest assured it won't be Batman killing him. If anyone would kill the Joker it would be one of the villians posssibly Jason Todd if he is in the game.

Nope.

They won't kill the Joker. They will just not have him in another game.

@Ahmad_Metallic said:

Batman villains don't ever die do they ?

Well, I guess it depends on how you walk away from Batman: The Killing Joke.

@bonorbitz said:

@Ahmad_Metallic said:

Batman villains don't ever die do they ?

Well, I guess it depends on how you walk away from Batman: The Killing Joke.

Too soon.

I don't think we'll see any villains die in game, least of all Joker.

Right. And I guess since Heath Ledger died, gives you another reason behind the thought of him dying as well. No, no one in the history of comics, villian or hero, has ever died; if they have, they came back. This isn't the end of Batman, so it's not the end of Joker.

Off the biggest Batman villain just because the voice actor is leaving? I don't see it happening.

@bonorbitz said:

@Ahmad_Metallic said:

Batman villains don't ever die do they ?

Well, I guess it depends on how you walk away from Batman: The Killing Joke.

Too soon.

@august said:

@bonorbitz said:

@Ahmad_Metallic said:

Batman villains don't ever die do they ?

Well, I guess it depends on how you walk away from Batman: The Killing Joke.

Too soon.

Goddammit, it took me a minute to get that. Followed.

@Underachiever007 said:

@august said:

@bonorbitz said:

@Ahmad_Metallic said:

Batman villains don't ever die do they ?

Well, I guess it depends on how you walk away from Batman: The Killing Joke.

Too soon.

Goddammit, it took me a minute to get that. Followed.

Joker shot (and some think raped) Batgirl and now she's paralyzed. That's the joke. 
 
Anyway, I dunno why people are all "No they can't kill off Batman's biggest villain in the game!". It's a game, not the comic. They can do whatever they want and it doesn't matter at all.