I know the whole thing from the Super Mario Bros. 2 instruction manual has been around for a while and I'd be tempted to say that a lot of the proof for Birdo being male is invalid as it is mostly all instruction manuals and promotional materials which are usually considered non-canon, but the fact that Birdo has been referred to as male so consistently does raise some questions. Honestly I think ambiguous is the exact right word to describe Birdo's gender and the Super Smash Bros. trophy is proof that this is definitely something that Nintendo recognise. I have a sneaking suspicion that Nintendo's more recent reluctancy to refer to Birdo as male or female may be them trying to dodge getting dragged into a whirlpool of strange gender politics over a character in their most popular franchise and what is supposed to be a "family" franchise, especially with their recent success with the Wii.
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