Bridget

In the Guilty Gear series, the character Bridget was the second of two twin boys born in a village where having twins of the same sex is considered to be a bad omen. Therefore, in order to protect him, his parents told the village that he was a girl, dressing and raising him appropriately, ultimately giving Bridget a very effeminate personality. Bridget isn't actually gay, and he identifies as a male, he just acts effeminate because of this background. While he does state he wants to be more masculine and refers to himself as a he, he seems to be totally unaware that his appearance and personality - not to mention the fact that despite long since having left his village, he still wears a nun's habit - still make him look very feminine.

The series' creator and character designer has stated that the Bridget character was originally designed to be a girl, but that he thought she wouldn't be interesting enough that way, and decided to make her a crossdressing boy instead.