Depends on how serious you are. If you are trying to make a serious website, I would recommend any of the below options--
ASP.NET- This is my personal favorite. The only downside is it is from Microsoft, so it requires a Windows server to host it.
Django - Giant Bomb was built on this web framework, so you are familiar with what it is capable of. It does sadden me that they are moving away from Django, as I believe it is the web framework with the brightest future.
Ruby on Rails - Even though I don't care much for it, you cannot argue with popularity. Twitter is one of the largest websites on this framework.
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