These two things stuck out to me as the strangest parts of this game, yet I haven't really heard anyone really talk that much in depth about them.

Why HOTEL? Does anyone think there's any meaning behind it? It so strange... especially when you come back to it later and the letters are huge and messed up. I've heard theroies that this game is this boys life presented to him through limbo, and it makes me think something significant happened to him at a hotel, something bad.

A little while after encountering the hotel sign after the first time, a mind slug takes control of you and makes you go very deep and to the right (past the elevator you must pull the bottom of the platform of to solve a puzzle). While your down there, it looks like your surrounded by gargantuan spider legs. It wigged me out so bad at first, thinking I was going into a huge spider liar. But they never move or anything. I haven't heard anyone talk about this. Maybe this, combined with the factory, is to show that the spiders were created with this boys fear?    

 Durden my man! I thought you left GB, I love your let's plays, your new Viewtiful Joe LP is funny as hell. 
 
Anyways, Limbo is very abstract, you should make of it whatever you like, it's pretty hard to find a definitive answer for what that game is trying to say.

I thought it was very interesting that this Hotel sign was the only text or words in the whole game.

Personally, I found that the first half of the narrative made no sense with the second half. 
 
The world was starting to evolve, technology was becoming more advanced, and you even discovered a race of people that had their subtle history being revealed to you--and then you played around with some gears and gravity till you found your sister. 
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Lying spiders are the WORST kind of spiders, in my experience.

"  Also, has anyone noticed how many tires are in this game? It adds more substance to the car accident idea.     "

I like the car accident idea, especially thinking about the tires. They threw me off a little, like, WTF are tires doing here?
 
A part that really stood out to me as some sort of symbolism was when you're underground, after first catching glimpses of the weird tribe people, and then a loud, horn-like noise sounds, you're standing in a pool of light and a dead tribe member is hanging with his arms out, pointing straight at you. (If this isn't ringing a bell, after you jump over the pool of water, there are three tribe members with blowguns that you need to escape from at this point.)
 
Gave me the willies, and he has a similar pose to the boy at the very end as he is slow-motion crashing through the "glass".

i dunno 'bout you guys but I thought Limbo was "The Adventures of Young Nathan Drake" coz of the the lil' kid's hair silhouette.