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Mary Holsom is a character in Duke Nukem Forever. She has a twin sister (who is unnamed). Together, the twins are Christian pop singers; an ironic choice of vocation, as the twins are also depicted as being slutty and sexually insatiable. Much like other female characters in Duke Nukem Forever, Mary has no real personality to speak of. She spends most of the game giggling and giving sexual favors to Duke. She also enjoys making out with her twin sister. She wears minimalist clothing and mostly yells out sex jokes.

Mary and her sister are eventually both kidnapped by aliens as part of a scheme to steal all of Earth's women. They are then put into pods by the impregnators. When Duke discovers her sordid fate, he acknowledges that she and her sister are "fucked" seconds before they blow up.

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