Final battle against Vergil at the bottom of the Bloody Palace in DMC 3. He kicked my ass, but after fighting him for a solid 30 minutes on top of traversing the 9,998 floors before him, I didn't give a damn that he beat me. I threw everything I had into that fight and came so close to winning I couldn't be upset.
Have to say, though, the boss battles against Jeanne in Bayonetta were alot more frantic and stylish, so they're at least tied.
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