Charlie MacDonald is an assassin ranked 25th by the UAA. The girls on his cheerleading squad (that Travis refers to collectively as Charlie's "hos") each occupy slots 26-49 each on the same list. Not much else is known other than the fact that Charlie is the star quarterback of Destroy University's football team, and that he is so attractive that girls just seem to flock to him. Together, Charlie and the cheerleaders can summon and pilot the Santa Death Parade, a giant laser-firing robot.
When Travis defeats him in battle, the robot containing the assassins flies into space to escape. Travis pursues them in his own mech, then obliterates the robot with a giant beam cannon, immediately jumping to rank 25. Charlie and the cheerleaders are never heard from again.
Yuri Lowenthal is the actor who voices Charlie MacDonald in No More Heroes 2.