The credit sequence at the funeral starts and you notice the screen pulsing in and out of focus. Well, this is a subtle hint that 47 isn't dead, he just had the drug that feigns death in his system, which explains why in the cutscene prior Diana put on "lipstick" before kissing him - she actually administered the antidote to increase his heartrate back up to normal levels (something that was foreshadowed in the rehab level where you administer it to someone in order to smuggle them out).
If you let the credits roll all the way through without doing anything, 47 is lowered into the grave and the game ends naturally. However, if you press the movement keys or analog stick enough he will wake up and you get a few seconds of dual hardballer pistol slo-mo to get your bearings and commence an epic shootout with the man who was hunting you and his goons.
When I figured it out for the first time, it left such an impact on me and is still one of my favorite endings of any game. The setup, the hinting to the player, and the payoff are all beautifully done.
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