The blue serum that turned Emil Blonsky (Tim Roth) into a super soldier references Captain America, the first super soldier created by the US government during World War II. Also,Blonsky's super quickness and agility coupled with his military training and experience demonstrated how Captain America would fare against a much stronger opponent like the Hulk
When Tony is flying the Mark 2 and decides to see how high he can go you can see on his HUD that the altitude record is held by the Lockheed SR-71 "Blackbird". The Blackbird is the name of the X-men's Jet from the comic and some incarnations of it are modeled after the SR-71
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