Mister Negative (the inverted colour, black and white guy from the Spider-man footage) isn't actually an original villain created for the game like Ben said, he's a more recent comic bad guy (well years old now he appeared after the whole One More Day arc but compared to the rest who show up) but not a new creation for this game.
I think the new villain is the one seen at the end of the trailer who isn't revealed to the viewer, though maybe he's a classic character turned villain judging by Spider-man's reaction. Anyway just a little correction for people.
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