Tiny Toon Adventures: Buster Busts Loose is a game based on the popular animated series of the same name. The game features several characters from the cartoon, including Buster, Plucky, Babs, Dizzy and Hampton J. Pig. The player assumes the role of Buster Bunny. As Buster, the player can dash and jump through six stages based off of episodes from the TV show. These stages include the halls of ACME University, catching a ride on a wild train, jumping though the skies, play the last hope for ACME University football team, exploring a haunted mansion and battling it out in space.
The game has variable levels of difficulty which change the number of lives, heart containers (health) and continues the player begins with. The easiest setting, Children, allows the player to continue as often as they'd like, but also removes a lot of the game's content including its ending and the entire sky stage (Stage 5). After each stage, the player spins a wheel to determine which of five bonus games they are to play for a chance to earn some extra lives.