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Introduced to the Mario series with Doki Doki Panic, Birdo is an effeminate dinosaur with a penchant for cute things. Although initially a boss enemy, nowadays she mostly appears as a friendly side character.
Bowser is one of the most iconic villains in gaming. His role as the main antagonist in the Mario franchise has cemented this King of Koopas' status in video game history.
The protagonist of the Double Fine game Stacking. A matryoshka doll on a quest to reunite his family.
The taller of the two frog bosses of the Clip Joint Calamity level of Inkwell Isle One in Cuphead
The Original Donkey Kong made his debut in the game of the same name, where he tossed barrels at his nemesis Mario. His grandson, Donkey Kong Jr., went on to create a legacy of his own in games such as Donkey Kong Country and Donkey Kong Country Tropical Freeze.
Protagonist of the Leisure Suit Larry games, originally created by Al Lowe for Sierra On-Line. He's constantly in search for some good loving but usually, thanks to his "charm" and "wit" fails miserably at it. Uncle of Larry Lovage.
Luigi is Mario's brother. He began as a simple palette swap of Mario, but was later differentiated. In his later forays, he provides comic relief.
Luigi's love interest and Peach's sidekick. She's the princess of Sarasaland.
Princess Peach Toadstool is usually the damsel in distress from the Mario franchise. She gets kidnapped frequently by Bowser, though she's not averse to being captured by various other villains from time to time.
The shorter of the two frog bosses of the Clip Joint Calamity level of Inkwell Isle One in Cuphead
Known as the "ultimate predator," Sam Fisher is the protagonist of the Splinter Cell franchise.
The Toad is a member of the Toad species. He is a loyal attendant of Princess Peach and often assists Mario on his journeys in a variety of ways.
Toadette is Toad's female counterpart and most commonly appears the various Mario spin-off games, such as the Mario Party and Mario Kart games.
Luigi's dark counterpart, who is mainly known for appearing in Mario spinoffs. He is voiced by Charles Martinet. Enjoys playing a game of tennis with Wario.
Wario is Mario's rival and doppelganger; a crude garlic-loving brute with an obsession with treasure. Wario's name is a portmanteau of the word "warui" ('bad' in Japanese) "Mario".
Yoshi is a recurring character in the Mario franchise and has since gone on to star in his own games.
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