In the main story, Lottie is simply but an average teddy bear. She is carried by Natalia Korda during the Claire Redfield portion of the story, though inevitably loses it once she is captured by Neil Fisher (who was the one who originally gave her the teddie years before at that) and sent to Alex Wesker. During Barry Burton's segment of the story, to which Natalia follows as a fellow playable protagonist, Natalia finds Lottie after having been torn apart by Alex.
In the bonus episode Little Miss, Lottie is instead portrayed as a living being, due to the episode being set within Natalia's own psyche. The episode tasks Natalia in finding postcards left by Lottie in order to locate her, however upon finally finding her Lottie responds aggressively and calls Natalia a bitch before being shown to revert to its original state as a teddy bear.
Lottie was also eventually added as a free costume for the Cipher character for Revelations 2's Raid Mode. She has her own series of voice samples as well, recycled from the Little Miss episode, though they are completely unrelated to the commands players select for her to say. Another Lottie costume was added too, this time having Lottie more closely resemble a polar bear.
- A DLC costume for Natalia has her cosplay as Lottie, as shown to the right.